Select this check box if you don't want the colored rectangle around the captured window to be shown when you point your cursor at it (the colored rectangle will not be present on the captured screenshots regardless of the state of this check box).
If this check box is selected, then you can press and hold the right Ctrl button to lock the cursor in certain position (e.g. over a certain button to activate its pop-up tooltip). Then, you can continue selecting windows/controls to be captured with another "virtual" cursor.